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教育活動は、２学年前期に興奮性組織の生理学と神経の生理学、２学年後期に循環・呼吸の生理学と口腔生理学を講義し、また２学年後期に口腔生理学実習を行っている。
研究活動としては、
（１）心筋および咀嚼筋（骨格筋）の動力学特性と可塑性の解析、筋疾患の病因の解明
（２）感覚情報処理に関与する中枢神経回路の発達と異常
（３）食品咀嚼時のヒトの咀嚼行動と嚥下誘発との関係の解析
などを進めている。
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| ・教授 |
三枝木 泰丈 |
＜担当科目：循環・呼吸の生理学、生理学実習＞ |
| ・講師 |
塩澤 光一 |
＜担当科目：口腔生理学、生理学実習＞ |
| ・講師 |
大貫 芳樹 |
＜担当科目：興奮性組織の生理学、生理学実習＞ |
| ・助教 |
本谷 安正 |
＜担当科目：神経の生理学、生理学実習＞ |
| ・教務員 |
篠田 陽子 |
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| ・大学院生 |
水木 雄亮 （高齢者歯科学） |
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| (1) |
心筋および咀嚼筋（骨格筋）の動力学特性と可塑性の解析、筋疾患の病因の解明： |
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筋の動力学特性についてはスキンド標本レベルでその筋長－張力－短縮速度－ATPase活性関係などを独自に開発した装置にて解析し、また筋の可塑性、筋肥大、筋疾患などについては、分子生物学的手法なども併用して、その発現機序の解明に努めている。（三枝木、大貫） |
| (2) |
食品咀嚼時のヒトの咀嚼行動と嚥下誘発との関係の解析： |
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食品咀嚼時のヒトの咀嚼行動と嚥下誘発との関係については、咀嚼の各相での食塊物性計測と咀嚼筋筋電図を記録して解析しており、『摂食・嚥下障害』に対する基礎研究である。（塩澤、水木 ） |
| (3) |
中枢神経回路における三量体Gタンパク質の機能解析： |
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電気生理学、分子生物学および発生工学的手法を用いて、三量体Gタンパク質の活性調節因子群の機能解析に努めている。（本谷） |
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過去５年間(2005～)の主要な著書・論文 |
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■著書 |
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| 1 |
第2章 血液, 第4章 心臓. 「人体機能生理学 改訂５版」. 杉 晴夫 編（分担執筆）, 南江堂, pp295-324, pp351-381.
三枝木泰丈 (2009) |
| 2 |
シナプス伝達、筋の収縮. 「基礎歯科生理学 第５版」. 森本俊文、山田好秋 編（分担執筆）, 医歯薬出版, pp45-57.
三枝木泰丈 (2008) |
| 3 |
「イラストでわかる歯科医学の基礎」 永末書店 （分担執筆）
塩澤光一 (2007) |
| 4 |
「咀嚼の本 －噛んで食べることの大切さ－」 日本咀嚼学会編, 口腔保健協会 （共著）
塩澤光一 (2006) |
| 5 |
筋電図. 「食感創造ハンドブック」 第二部：食感評価技術, 第三章：新しい評価技術, 第四節, サイエンスフォーラム
塩澤光一 (2005) |
| 6 |
Gα12/13共役GPCRによるRho活性化機構. 「医学のあゆみ」 Vol.212, 医歯薬出版, pp17-20.
本谷安正、小笹 徹 |
| 7 |
Measurement of force developed by a single cardiac myocyte using novel carbon fibers. In Molecular and Cellular Aspects of Muscle Contraction; Edited by Sugi H, Kluwer Academic Plenum Publishers, pp381-387.
Sugiura S, Yasuda S, Yamashita H, Kato K, Saeki Y, Kaneko H, Suda Y, Nagai R & Sugi H. (2004) |
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■論文 |
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Kobayashi N, Kohyama K, Shiozawa K. Fragmentation of a viscoelastic food by human mastication. J Physic Soc Jap 79(4): 044801/1-4, 2010. |
| 1 |
Effects of increased occlusal vertical dimension on daily activity and myosin heavy chain composition in rat jaw muscle. Arch Oral Biol 54: 783-9,
Ohnuki Y, Kawai N, Tanaka E, Langenbach GEJ, Tanne K, Saeki Y (2009) |
| 2 |
Phosphorylation status of regulatory proteins and functional characteristics in myocardium of dilated cardiomyopathy of Sylian hamsters. J Physiol Sci 58: 15-20.
Ohnuki Y, Nishimura S, Sugiura S, Saeki Y (2008) |
| 3 |
Jaw-opening muscle contracts more economically than jaw-closing muscle in rat. Arch Oral Biol 53: 193-8.
Ohnuki Y, Saeki Y (2008) |
| 4 |
成人被験者における唾液分泌量が咀嚼時の食塊物性変化に及ぼす影響. 老年歯科医学 22: 288-97.
住野広明、塩澤光一、森戸光彦 (2008) |
| 5 |
口どけ感のあるビスケットの物性特性. 日咀嚼誌 18: 112-20.
宅見央子, 中村弘康, 福田真一, 水木雄亮, 住野広明, 白石浩荘, 米谷 俊, 森戸光彦, 塩澤光一 (2008) |
| 6 |
嚥下閾値に及ぼすトロミ剤の影響. 日咀嚼誌 17: 27-34.
塩澤光一、神山かおる、柳沢慧二 (2007) |
| 7 |
Phenotypic and genetic characterization of the Motohm mottled mouse: a new murine model of Menkes disease. Genomics 87: 191-9.
Mototani Y, Miyoshi I., Okamura T, Moriya T, Meng Y, Pei XY, Kameo S, Kasai N (2006) |
| 8 |
Microtubules modulate the stiffness of cardiomyocytes against shear stress.
Circ Res 98: 81-7.
Nishimura S, Saeki Y, Nagai R, Sugiura S (2006) |
| 9 |
Expression of green fluorescent protein impairs the force-generating ability
of isolated rat ventricular cardiomyocytes. Mol Cell Biochem 286: 59-65.
Nishimura S, Nagai S, Sata M, Katoh M, Yamashita H, Saeki Y, Nagai R, Sugiura S (2006) |
| 10 |
Transverse stiffness of myofibrils of skeletal and cardiac muscles studies
by atomic force microscopy. J Physiol Sci 56: 145-51.
Akiyama N, Ohnuki Y, Kunioka Y, Saeki Y, Yamada T (2006) |
| 11 |
Effects of clenbuterol and cyclosporin A on the myosin heavy chain mRNA level and the muscle mass in rat masseter. J Physiol Sci 56: 205-9.
Arai C, Ohnuki Y, Umeki D, Hirashita A, Saeki Y (2006) |
| 12 |
IImpaired Ca2+ store functions in skeletal and cardiac muscle cells from sarcalumenin-deficient mice. J Biol Chem 280: 3500-6.
Yoshida M, Minamisawa S, Shimura M, Komazaki S, Kume H, Zhang M, Matsumura K, Nishi M, Saito M, Saeki Y, Ishikawa Y, Yanagisawa T, Takeshima H (2005) |
| 13 |
Effects of bite-opening and cyclosporine A on the mRNA levels of myosin heavy chain and the muscle mass in rat masseter. Jpn J Physiol 55: 173-9.
Arai C, Ohnuki Y, Umeki D , Saeki Y. (2005) |
| 14 |
Contractile dysfunction of cardiomyopathic hamster myocytes is pronounced
under high load conditions. J Mol Cell Cardiol 39: 231-9.
Nishimura S, Yamashita H, Katoh M, Yamada KP, Sunagawa K, Saeki Y, Ohnuki Y, Nagai R, Sugiura S. (2005) |
| 15 |
米飯咀嚼時の食塊物性変化. 日咀嚼誌 15: 37-42.
塩澤光一、神山かおる、柳沢慧二 (2005) |
| 16 |
Role of Ca2+ in determining the rate of tension development and relaxation in rat skinned myocardium. J Mol Cell Cardiol 36: 371-80.
Saeki Y, Kobayashi T, Yasuda S, Nishimura S, Sugiura S, Yamashita H, Sugi H (2004) |
| 17 |
Single cell mechanics of rat cardiomyocytes under isometric, unloaded,
and physiologically loaded conditions. Am J Physiol 287: H196-202.
Nishimura S, Yasuda S, Katoh M, Yamada KP, Yamashita H, Saeki Y, Sunagawa K, Nagai R, Hisada T, Sugiura S (2004) |
| 18 |
Effect of deuterium oxide on contraction characteristics and ATPase activity
in glycerinated single rabbit skeletal muscle fibers. Biochim Biophys Acta 1659: 46-51.
Kobayashi T, Saeki Y, Chaen S, Shirakawa I , Sugi H (2004) |
| 19 |
Restoration of copper metabolism and rescue of hepatic abnormalities in LEC rats, an animal model of Wilson disease, by expression of human ATP7B gene. Biochim Biophys Acta 1690: 208-19.
Meng Y, Miyoshi I, Hirabayashi M, Su M, Mototani Y, Okamura T, Terada K, Ueda M, Enomoto K, Sugiyama T, Kasai N (2004) |
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2003年以前の主要な著書・論文 |
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■著書 |
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| 1 |
シナプス伝達、筋の収縮. 「基礎歯科生理学 第４版」 中村嘉男、森本俊文、山田秋好編, 医歯薬出版, pp44-56.
三枝木泰丈 (2003) |
| 2 |
Myosin heavy chain isoforms modulate motor function of cardiac myosin by changing crossbridge kinetics.
In Pathophysiology of Cardiovascular Diseases (Progress in Experimental Cardiology, 10), Eds. Dhalla NA, et al., Kluwer Academic Pub, pp35-50.
Yamashita H, Sugiura S, Fujita H, Yasuda S, Nagai R, Saeki Y, Sugi H.(2003) |
| 3 |
筋電図による咀嚼行動と食品物性の関係解析. 「老化抑制と食品＝抗酸化・脳・咀嚼＝」 第４章：咀嚼機能の老化と食品, 第７節, 食品総合研究所編, IPC出版, pp362-377.
塩澤光一 (2002) |
| 4 |
「スタディ口腔生理学」 永末書店.
塩澤光一 (2001) |
| 5 |
Fiber types and their functional role in mammalian masticatory muscles. In: Neurobiology of Mastication---from Molecular to Systems Approach, Eds. Nakamura Y & Sessle BJ, Elsevier, 327-36.
Saeki Y, Ohnuki Y, Yamane A, Yanagisawa K (1999) |
| 6 |
Influences of protein kinase A and D-cAMP on actin-myosin interaction and energy consumption of cardiac muscles.
In Mechanisms of Work Production and Work Absorption in Muscle., Eds. Sugi H & Pollack GH, Plenum Press, pp461-470.
Saeki Y, Kobayashi T, Takigiku K, Sugi H (1998) |
| 7 |
筋の収縮. 「基礎歯科生理学 第３版」 中村嘉男、森本俊文編, 医歯薬出版, 45-53.
三枝木泰丈 (1998) |
| 8 |
Oral Motor Function and Dysfunction. Increase in vertical dimension alters myosin isozymes in guinea pig masseter muscles. In: Brain and Oral Functions, Elsevier, pp435-438.
Saeki Y, Fukasawa S, Imamura S, Yanagisawa K, Kuwahara Y (1995) |
| 9 |
Tension and intracellular calcium transients of activated ferret ventricular muscle in response to step length changes.
In Mechanism of Myofilament Sliding in Muscle Contraction, Plenum Press, pp639-648.
Saeki Y, Kurihara S, Hongo K, Tanaka E (1993) |
| 10 |
心筋の収縮と弛緩.「心臓の適応と制御」朝倉書店, pp29-38.
三枝木泰丈 (1992) |
| 11 |
Effects of intracellular acidification on Ca2+ transients and contraction in mammalian cardiac muscles. In: The Diabetic Heart, Raven Press, pp515-522.
Kurihara S, Tanaka E, Hongo K, Suda N, Okazaki O, Saeki Y (1991) |
| 12 |
Isotonic segment dynamics of isolated cardiac muscle. In Cardiac Mechanics and Function in the Normal and Diseased Heart, Springer-Verlag, pp35-44.
Saeki Y, Horikoshi T, Shiozawa K (1989) |
| 13 |
X-ray diffraction study on heart muscle during contraction. In Muscle Energetics, Alan R. Liss, pp481-486.
Matsubara I, Yagi N, Maughan DW, Saeki Y, Amemiya Y (1989) |
| 14 |
Visco-elasticity of heart muscle studied by perturbation methods. In New Approaches in Cardiac Mechanics, Japan Scientific Societies Press, Gorden and Breach Science Publishers, pp57-67.
Saeki Y, Totsuka T (1986) |
| 15 |
Transient length responses of heart muscle in Ba2+-contracture to step tension reductions. In Contractile Mechanisms in Muscle, Plenum Press, pp841-843.
Saeki Y, Shibata T, Sato C, Yanagisawa K (1984) |
| 16 |
心筋活動電位．「心臓生理学」学会出版センター, pp225-252.
三枝木泰丈 分担訳 (1981) |
| 17 |
心筋の動的硬さと収縮機構．「バイオメカニズム５－動体の機構と制御－」東京大学出版会, pp28-35.
三枝木泰丈、柴田利満 (1980) |
| 18 |
Possible mechanism of rate-dependent change of contraction in dog ventricular muscle: Relation to calcium movements. In Recent Advances in Studies on Cardiac Structure and Metabolism, Volume 11 (Heart Function and Metabolism), University Park Press, Baltimore, pp131-135.
Saeki Y, Kamiyama A (1979) |
| 19 |
Dynamic stiffness of heart muscle in twitch and contracture. In Cross-Bridge Mechanism in Muscle Contraction, University of Tokyo Press, pp171-190
Sagawa K, Saeki Y, Loeffler III L, Nakayama K (1978) |
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■論文 |
|
| 1 |
Relationship between physical properties of a food bolus and the swallowing threshold during mastication of gel type food. Jpn J Oral Biol 44: 114-9.
Shiozawa K, Koyama K, Yanagisawa K (2003) |
| 2 |
Relationship between physical properties of a food bolus and initiation
of swallowing. Jpn J Oral Biol 45: 59-63.
Shiozawa K, Kohyama K, Yanagisawa K (2003) |
| 3 |
Unloaded shortening increases peak of Ca2+ transients but accelerates their
decay in rat single cardiac myocytes. Am J Physiol 285: H470-5.
Yasuda S, Sugiura S, Yamashita H, Nishimura S, Saeki Y, Momomura S, Katoh K, Nagai R, Sugi H. (2003) |
| 4 |
Ectopic expression of an embryonic skeletal myosin heavy chain in human
fetal and Syrian hamster hearts. J Muscle Res Cell Motil 24: 399-406.
Minamisawa S, Hiratsuka E, Ruiz-Lozano P, Machida S, Furutani Y, Nishimura M, Takao A, Yanagisawa K, Momma K, Saeki Y, Matsuoka R. (2003) |
| 5 |
Myosin light chain isoforms modify force-generating ability of cardiac
myosin by changing the kinetics of actin-myosin interaction. Cardiovasc Res 60: 580-8.
Yamashita H, Sugiura S, Fujita H, Yasuda S, Nagai R, Saeki Y, Sunagawa K, Sugi H (2003) |
| 6 |
Bilateral congenital cataracts result from a gain-of-function mutation in the gene for aquaporin-0 in mice. Genomics 81: 361-8.
Okamura T, Miyoshi I, Takahashi K, Mototani Y, Ishigaki S, Kon Y, Kasai N (2003) |
| 7 |
Relationship between physical properties of a food bolus and the swallowing
threshold during mastication of gel type food. Jpn J Oral Biol 44: 114-9.
Shiozawa K, Kohyama K, Yanagisawa K (2002) |
| 8 |
咀嚼時の唾液分泌量の増加が嚥下誘発に及ぼす影響. 日咀嚼誌 11: 117-21.
塩澤光一、神山かおる、柳沢慧二 (2002) |
| 9 |
Radial mass transfer of cross-bridges in a tetanized ferret muscle. Pflugers Arch 444: 38-42.
Yagi N, Saeki Y, Kiyota H, Kurihara S (2002) |
| 10 |
Effects of diet consistency on the myosin heavy chain mRNAs of rat masseter muscle during postnatal development. Arch. Oral Biol 47: 109-15.
Saito T, Ohnuki Y, Yamane A, Saeki Y (2002) |
| 11 |
Postnatal changes in the nicotimic acetylcholine receptor subunits in rat
masseter muscle. Arch. oral Biol 47: 417-21.
Saito T, Ohnuki Y, Saeki Y, Nakagawa Y, Ishibashi K, Yanagisawa K, Yamane A (2002) |
| 12 |
Mouse transgenic for murine oviduct-specific glycoprotein promoter-driven
simian virus 40 large T-antigen: tumor formation and its hormonal regulation.
Mol Reprod Dev 63: 168-76.
Miyoshi I, Takahashi K, Kon Y, Okamura T, Mototani Y, Araki Y, Kasai N (2002) |
| 13 |
Protein kinase A increases the rate of relaxation but not the rate of tension
development in skinned rat cardiac muscle. Jpn J Physiol 51: 427-33.
Saeki Y, Takigiku K, Iwamoto H, Yasuda S, Yamashita H, Sugiura S, Sugi H (2001) |
| 14 |
Cross-bridge and calcium behavior in ferret papillary muscle in different thyroid states. Jpn J Physiol 51: 319-26.
Yagi N, Saeki Y, Ishikawa T, Kurihara S (2001) |
| 15 |
A novel method to study contraction characteristics of a single cardiac
myocyte using carbon fibers. Am J Physiol 281: H1442-6.
Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, Sugi H (2001) |
| 16 |
Developmental changes in the nicotinic acetylcholine receptor in mouse
tongue striated muscle. J Dent Res 80: 1840-4.
Yamane A, Ohnuki Y, Saeki Y (2001) |
| 17 |
Quantitative changes in the mRNA for contractile protein and metabolic enzymes in masseter muscle of bite-opened rats. Archs oral Biol 45: 1025-32.
Ohnuki Y, Saeki Y, Yamane A, Yanagisawa K (2000) |
| 18 |
Expression of myogenic regulatory factors during the development of mouse
tongue striated muscle. Archs oral Biol 45: 71-8.
Yamane A, Mayo M, Shuler C, Crowe D, Ohnuki Y, Dalrymple K, Saeki Y (2000) |
| 19 |
Delayed embryonic development of mouse masseter muscle correlates with
delayed MyoD family expression. J Dent Res 79:1933-6.
Yamane A, Ohnuki Y, Saeki Y (2000) |
| 20 |
Influence of ingested food texture on jaw muscle and tongue activity during mastication in humans. Jpn J Oral Biol 41: 27-34.
Shiozawa K, Kohyama K, Yanagisawa K (1999) |
| 21 |
Food Bolus Texture and Tongue Activity Just before Swallowing in Human
Mastication. Jpn J Oral Biol 41: 297-02.
Shiozawa K, Kohyama K, Yanagisawa K (1999) |
| 22 |
Adaptation of guinea-pig superficial masseter muscle to an increase in
occlusal vertical dimension. Archs oral Biol 44: 329-35.
Ohnuki Y, Saeki Y, Yamane A, Kawasaki K, Yanagisawa K (1999) |
| 23 |
Role of microtubules in the viscoelastic properties of isolated cardiac muscle. J Mol Cell Cardiol 30: 1841-53.
Yamamoto S, Tsutsumi H, Takahashi M, Ishibashi Y, Tagawa H, Imamura-Yoshida
K, Saeki Y, Takeshita A (1998) |
| 24 |
Dynamic relations among length, tension, and intracellular Ca2+ in activated
ferret papillary muscles. Am J Physiol 275: H1957-62.
Saeki Y, Kurihara S, Komukai K, Ishikawa T, Takigiku K (1998) |
| 25 |
Electromyographic study on cooked rice with different amylose contents.
J Texture Studies 29: 101-13.
Kohyama K, Ohtsubo K, Toyoshima H, Shiozawa K (1998) |
| 26 |
Protein kinase A increases the tension cost and unloaded shortening velocity in skinned rat cardiac muscle. J Mol Cell Cardiol 29: 1655-63.
Saeki Y, Kobayashi T, Minamisawa S, Sugi H (1997) |
| 27 |
Effect of an increase in occlusal vertical dimension on the rate of cyclic
actin-myosin interaction in guinea-pig masseter muscle. Archs oral Biol 42: 505-12.
Kawasaki K, Saeki Y, Ohnuki Y (1997) |
| 28 |
Crossbridge dynamics under various inotropic states in cardiac muscle:
evaluation by perturbation analyses. Jpn J Physiol 45: 687-705.
Saeki Y (1995) |
| 29 |
Comparison of mechanical characteristics between segments and whole excised cardiac muscle by means of new servo-control system. Med & Biol Eng & Comput 32: S60-4.
Saeki Y, Yanagisawa K, Shibayama H (1994) |
| 30 |
Alterations in intracellular calcium and tension of activated ferret papillary
muscle in response to step length changes. J Physiol 463: 291-306.
Saeki Y, Kurihara S, Hongo K, Tanaka E (1993) |
| 31 |
Crossbridge scheme and the kinetic constants of elementary steps deduced
from chemically skinned papillary and trabecular muscles of the ferret. Circ Res 73: 35-50.
Kawai M, Saeki Y, Zhao Y (1993) |
| 32 |
Increase in vertical dimension alters mechanical properties and isometric ATPase activity in guinea pig masseter. Am J Orthod Dentofac Orthop 104: 484-91.
Paik CH, Satomi M, Saeki Y, Yanagisawa K, Kuwahara Y (1993) |
| 33 |
顎筋の特性．神経進歩37：845-852.
三枝木泰丈 (1993) |
| 34 |
Hyperthyroid dog left ventricle has the same oxygen consumption versus
pressure-volume area (PVA) relation as euthyroid dog. Heart Vessels 6: 71-83.
Suga H, Tanaka N, Ohgoshi Y, Saeki Y, Nakanishi T, Futaki S, Yaku H, Goto Y(1991) |
| 35 |
Activation dependence of isotonic transient in response to step tension
reduction in cardiac muscle segment during barium contracture. J Muscle Res Cell Motil 12: 152-60.
Saeki Y, Shiozawa K, Paik CH, Yanagisawa K (1991) |
| 36 |
Cross-bridge movement in fast and slow skeletal muscles of the chick. J Physiol 441: 113-20.
Matsubara I, Yagi N, Saeki Y, Kurihara S (1991) |
| 37 |
Comparison of crossbridge dynamics between intact and skinned myocardium
from ferret right ventricles. Circ Res 68: 772-81.
Saeki Y, Kawai M, Zhao Y (1991) |
| 38 |
Adrenaline increases the rate of cross-bridge cycling in rat cardiac muscle.
J Mol Cell Cardiol 22: 453-60.
Saeki Y, Shiozawa K, Yanagisawa K, Shibata T (1990) |
| 39 |
Isotonic myocardial segment dynamics during barium contracture as a useful index of the cross-bridge kinetics: effect of adrenaline: Frontiers Med Biol Engng 2: 195-9.
Saeki Y (1990) |
| 40 |
Effects of length change on intracellular Ca2+ transients in ferret ventricular
muscle treated with 2,3-Butanedione monoxime (BDM). Jpn J Physiol 40: 915-20.
Kurihara S, Saeki Y, Hongo K, Tanaka E, Sudo N (1990) |
| 41 |
Cross-bridge movement in rat cardiac muscle as a function of calcium concentration.
J Physiol 417: 555-65.
Matsubara I, Maughan DW, Saeki Y, Yagi N (1989) |
| 42 |
ATPase activity and tension development in mechanically-skinned feline jaw muscle. Archs oral Biol 32: 207-10.
Saeki Y, Kato C, Satomi M, Yanagisawa K (1987) |
| 43 |
Mechanical properties and ATPase activity in glycerinated cardiac muscle
of hyperthyroid rabbit. Pflugers Arch 408: 578-83.
Saeki Y, Kato C, Totsuka T, Yanagisawa K(1987) |
| 44 |
Length-tension relation of cat heart muscle studied by a segment-control
method. Heart Vessels 2: 208-12.
Saeki Y, Totsuka T, Kato C, Yanagisawa K (1986) |
| 45 |
Ca2+ sensitivities and transient tension responses to step-length stretches in feline mechanically-stripped single-fibre jaw-muscle preparations. Archs oral Biol 30: 429-32.
Kato C, Saeki Y, Yanagisawa K (1985) |
| 46 |
Transient tension responses of glycerinated guinea pig temporal and masseter
muscles to step length stretches. Jpn J oral Biol 26: 302-05.
Kato C, Saeki Y, Yanagisawa K (1984) |
| 47 |
Effects of Ba2+ on the mechanical properties of glycerinated heart muscle.
Pflugers Arch 400: 235-40.
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